ICSI

High quality micromanipulation pipettes ensure precise and effective sperm injection while reducing any damage to the oocyte. Oils and media are also essential in this step for correct gamete manipulation and stability.

Conventional IVF

For conventional IVF, oocytes are maintained in Fertilization media with Hypure™ Oil overlay and a small sperm aliquote is added in the same dish to bring the gametes closer and improve the chances of a successful fertilization.

ICSI

Is a micromanipulation technique that maximizes fertilization outcome specially in cases of poor sperm quality since one spermatozoon is directly introduced in the oocyte’s cytoplasm (1). For this, the surrounding cumulus cells need to be removed. Oocytes are exposed to Hyaluronidase to gently loose granulosa cells junctions and further denuded by mechanical stripping in Gamete Buffer droplets covered with Hypure™ Oil.

(1) Rubino P. et al. The ICSI procedure from past to future: a systematic review of the more controversial aspects Hum Reprod Update. Mar-Apr 2016;22(2):194-227.

ICSI

Is a micromanipulation technique that maximizes fertilization outcome specially in cases of poor sperm quality since one spermatozoon is directly introduced in the oocyte’s cytoplasm (1). For this, the surrounding cumulus cells need to be removed. Oocytes are exposed to Hyaluronidase to gently loose granulosa cells junctions and further denuded by mechanical stripping in Gamete Buffer droplets covered with Hypure™ Oil.

(1) Rubino P. et al. The ICSI procedure from past to future: a systematic review of the more controversial aspects Hum Reprod Update. Mar-Apr 2016;22(2):194-227.

Once in the ICSI a dish is prepared with Gamete Buffer and Hypure™ Oil, spermatozoa are handled and slowed with PVP, which ease loading of the selected spermatozoon into the ICSI Pipette. The oocyte will be stabilized with the Holding Pipette, the ICSI Pipette introduced through the zona pellucida and oolemma and finally the spermatozoon left safely inside the ooplasm.

Kitazato Hypure™ Oil aids to maintain temperature stability around 37ºC during oocyte injection, which is found to improve fertilization rates and embryo development.

Kitazato Holding Pipettes

Kitazato Holding Pipettes are available with different diameters to ease manipulation of different stages of development (oocytes to blastocysts) through the varied micromanipulation techniques. Their smooth polished tips guarantee a high level of control with no harm to the specimens handled.

Kitazato ICSI Pipettes

Kitazato ICSI Pipettes are available with short or long parallel walls and spiked tip to minimize damage to the oocyte, also offering a smooth handling of spermatozoa and ooplasm.

The IVF Lifecycle

Kitazato offers a well curated selection of quality products that maximize success at every step of the IVF Lifecycle. Learn more about the products involved in each IVF procedure.

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Oocyte Retrieval
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Andrology
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Culture
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Vitrification
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Embryo Transfer
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Oocyte Retrieval
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Andrology
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Culture
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Vitrification
More info
Embryo Transfer
More info