Vitrification and Thawing Media

Kitazato vitrification media are the most versatile option for cryopreservation in your laboratory.

Increase your efficiency by using the same media for vitrification and warming of oocytes and embryos, in all their stages of development, from zygote stage to blastocyst.

The Cryotop® Method offers the same products for both oocyte and embryo vitrification.

This helps to standardize procedures, optimize laboratory routine and improve clinical results.

VT601 Vitrification Media

  • 1 x 1.5 ml Basic Solution vial (BS)
  • 1 x 1.5 ml Equilibration Solution vial (ES)
  • 2 x 1.5 ml Vitrification Solution vial (VS)

VT602 Thawing Media

  • 2 x 4 ml Thawing Solution vial (TS)
  • 1 x 4 ml Diluent Solution vial (DS)
  • 1 x 4 ml Washing Solution vial (WS)

Kitazato media’s composition is entirely synthetic. Among their components, the following are notable: 

  • Hydroxypropyl cellulose (HPC) prevents the risk of contamination, increases the survival rate in hatched blastocysts and reduces mechanical stress during warming.
  • Trehalose functions as an osmotic agent in place of sucrose. It provides greater safety in the process, improving the protection of the cellular membranes.
  • DMSO in combination with ethylene glycol in the media assures less toxicity and the best outcomes after warming. 
  • The incorporation of gentamicin prolongs the sterility of the media, guaranteeing greater safety in handling the solutions. 

Kitazato media’s composition is entirely synthetic. Among their components, the following are notable: 

  • Hydroxypropyl cellulose (HPC) prevents the risk of contamination, increases the survival rate in hatched blastocysts and reduces mechanical stress during warming.
  • Trehalose functions as an osmotic agent in place of sucrose. It provides greater safety in the process, improving the protection of the cellular membranes.
  • DMSO in combination with ethylene glycol in the media assures less toxicity and the best outcomes after warming. 
  • The incorporation of gentamicin prolongs the sterility of the media, guaranteeing greater safety in handling the solutions. 

Kitazato media composition works effectively at room temperature, allowing convenient exchange between water and CPAs and preserving an intact cell membrane. 

The only step that needs to be performed at 37°C is the first one of warming procedure. The temperatures difference between the liquid nitrogen at -196°C and the first solution at 37°C is the warming rate, which is essential for the success of the procedure.

How to perform a succesfull vitrification

Embryo Vitrification and Thawing Protocol

Order Number Code Product Name Quantity
91101 VT601 Vitrification Media 4 x 1.5ml
91121 VT602 Thawing Media 4 x 4ml
91171 VT801 Vitrification Media 4 x 1.5ml
91182 VT802 Thawing Media 4 x 4ml
91103 VT601US Vitrification Media 4 x 1.5ml
91130 VT602US Thawing Media 4 x 4ml

Please note that products may only be available in selected countries.

  • pH tested
  • Osmolality tested
  • SAL (Sterility Assurance Level): 10-6
  • Endotoxin ≤ 0.25 EU/device
  • Mouse embryo assay (MEA)
  • Shelf life VT601 and VT602: 12 months
  • Shelf life VT801 and VT802: 6 months (available in selected countries)
  • Safety Data Sheet VT601 VT602
  • Safety Data Sheet VT801 VT802

  • HEPES buffer
  • Dimethyl sulfoxide (DMSO)
  • Ethylene glycol (EG)
  • Trehalose
  • Hydroxypropyl cellulose (HPC)
  • Gentamicin (only VT600)

Vitrification Product Catalog

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Vitrification Protocol

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Vitrification solution VT601 IFU

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Vitrification solution VT602 IFU

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Vitrification solution VT801 IFU

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Vitrification solution VT802 IFU

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